Genetic polymorphism of mitochondrial DNA hypervariable segment Ⅰof Mulam ethnic group in Guangxi

doi:10.3969/j.issn.0529-1356.2013.04.022

Abstract

Abstract:

Objective To demonstrate the polymorphism of the mitochondrial DNA (mtDNA) hypervariable segment Ⅰ(HVSⅠ) and to clearly understand the maternal genetic structure of Mulam ethnic group in Guangxi. Methods Venous blood samples from 91
unrelated Mulam individuals were collected. HVS I of mtDNA was amplified using primers L15947 and R16488. PCR amplicons were sequenced in both forward and reverse directions using the ABI 3730. Polymorphisms were scored relative to the revised Cambridge
reference sequence (rCRS). The number of polymorphic sites, number of haplotypes, the mean number of pairwise differences and genetic distances between populations were calculated. Polygenetic tree was drawn based on dA distance. Results MtDNA HVSⅠsequences
showed high levels of diversity. Seventy-four haplotypes were identified among the 91 samples. The nucleotide diversity was 0.0188±0.010 and the mean number of pairwise differences was 6.618±3.154. Genetic distances between populations and the cluster
map show that Mulam falls together with southern groups, but away from northern populations. Among southern populations, Mulam clusters were most closely with the Zhuang and Sui. Conclusion Mulam is a typical southern Kam-Tai population, and may experience
population expansion or selection. MtDNA HVS-Ⅰ reveals highly genetic diversities in Mulam, which may have potential use in population genetics and forensic practice.

Key words: Guangxi, mt DNA, HVS I, Sequencing, Genetic polymorphism, PCR, Mulam

WANG Xiao-qing WANG Chuan-chao DENG Qiong-ying* LI Hui . Genetic polymorphism of mitochondrial DNA hypervariable segment Ⅰof Mulam ethnic group in Guangxi[J]. AAS, 2013, 44(4 ): 559-562.

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